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Leica CM1850 UV Sectioning

Leica CM1850 UV Cryostat for Standard Applications in the Clinical Histopathology Laboratory

Leica CM1950 cryostat: Quick and effective disinfection with UVC

Leica Cryostats: Reduced Infection Risk

UVC disinfection effective for destroying clinically relevant pathogens

 

Chemical disinfectants are not only toxic for the user but are also awkward to use and dispose of. Leica Microsystems has therefore developed cryostats with UVC disinfection to enhance safety and efficiency when cutting cryosections. The method is simple to use and requires no warming of the cryochamber.

To prove the efficiency of UVC radiation, Dr. Ingo Maier, ecoscope, Laboratory for Microbiology and Ecotoxicology (Amtzell, Germany) carried out test series with Leica cryostats.

The results confirm the germicidal effect of UVC disinfection:

  • Disinfection after 30 to 40 minutes
  • UVC-resistant viruses inactivated

Leica CM1950 Clinical Cryostat

One Pathologist’s Approach to Teaching Frozen Sectioning

Anyone who has visited Dr. Peter’s website tutorial on frozen section technique knows that he is a true believer in the brush technique. Let’s learn how he teaches this valuable skill to his residents:

The first step is to find a comfortable hand position to maximize fine motor ability for this very delicate maneuver. For this, I have my students hold the brush like a pen and gently rest a finger on the anti-roll plate or at the edge of the cryostat stage.

Next, I tell them to try to write their names on the edge of the block with the brush to gain a sense of the fine movements they will use in the sectioning process.

To learn more about this pathologist’s approach to cryosectioning visit Dr. Peter’s tutorial at: http://pathologyinnovations.com/frozen_section_technique.htm.

Trimmed block face of colored sesame seeds

The Art of Embedding Tissue for Frozen Section

(by Stephen R. Peters, Dept. of Pathology, Hackensack University Medical Center, New Jersey, USA)

This article describes a system used for embedding of tissues for the preparation of frozen sections.

This novel system uses simple techniques and apparatus to accomplish face-down embedding in freezing-temperature steel wells.

The system is easy to learn and offers many advantages over conventional methods, including speed, high precision and predictability, and reduced tissue wastage.

The system is easily adapted in most existing cryostats, used no additional consumables, and requires minimal maintenance. (The J Histotechnol 26:11, 2003)

  • <media 25588 _blank download>Download the complete pdf</media>

Micrograph showing strips of skin embedded on edge

Paper Cryoembedding

(by Stephen R. Peters et al)

The article describes a unique method of face-down tissue embedding for frozen section in freezing-temperature well bars.

Tissue is first placed face down in a precise position on a piece of lens paper. The tissue is then transferred in the exact position to an embedding well.

The block is completed while the tissue remains face down on the paper. The paper is removed during the initial trimming of the block, leaving the tissue in its original position and orientation.

The technique accomplishes precise embedding of extremely delicate and deformable tissues and arrangements of tissues.

We believe this technique will facilitate a variety of difficult embedding tasks in areas of surgical pathology, Mohs surgery, and research settings
(The J Histotechnol 26:173, 2003).

  • <media 25587 _blank download>Download the complete pdf</media>