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Tutorials

Step-by-step instructions and tips for Histology and Anatomic Pathology professionals.

This article provides an overview of biopsy procedures with an example of observations in a comprehensive health network biopsy suite.

For routine diagnosis, the use of Hematoxylin and Eosin (H&E) is by far preferred for viewing cellular and tissue structure detail by pathologists. 

Though the H&E stain is a relatively simple stain to perform, there are a variety of artifacts that can interfere with a good stain. Artifacts can be attributed to a variety of causes.

After a joint has been decalcified, the loss of proteoglycans in the articular cartilage causes it to fold and ripple during the staining process. When this occurs, it makes it impossible to perform histo-morphometry or measurements of the cartilage.

Microorganisms are living organisms which include bacteria, fungi, protozoa and viruses. Bacteria, fungi and protozoa can be identified and classified with histochemical procedures and viruses are generally identified with immunohistochemical procedures.

Special stains" are processes that generally employ a dye or chemical that has an affinity for the particular tissue component that is to be demonstrated. This article includes the processes and protocols necessary to demonstrate connective tissue.

Mucins are a part of a complex group called carbohydrates. Mucins are mucopolysaccharides; they are important in cell growth as they help regulate the flow of nutrients between capillaries and cells and are known as “The Glue of Life”.

Muscular dystrophy is a genetic disease characterized by progressive weakness and degeneration of the skeletal muscles which controls movement. The muscles of the heart and some other involuntary muscles are also affected in some forms of muscular dystrophy.

Users of microtomes often ask if they can use just one microtome across all functions in the laboratory. This article addresses the advantages and limitations of Vibrating Blade microtomes.  

A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared.

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