The Power of Digital Pathology in Research 1. Leica Biosystems Proprietary Information. For Research Use Only. Not For Use in Diagnostic The Power of Digital Pathology In Research. Caitríona Lyons PhD. 2. Introduction • Caitríona Lyons PhD • Based in Dublin, Ireland • Current Role: • Product Manager Image Analysis, Leica Biosystems • Previous Roles: • Product Owner Image Analysis, Leica Biosystems • Image Analysis Specialist, Leica Biosystems • Education • BSc (Hons) Biomedical Science • PhD Medicine and Health • Postdoc Immunology 3. Agenda • History of Pathology • What is Digital Pathology • Power of Digital Pathology • Leica Biosystems Role in Digital Pathology • The Future of Digital Pathology • Final Thoughts 3 SCAN MANAGE ANALYZE 4. History of Digital Pathology • Patholog...

The first virtual event of the year recaptured the conference atmosphere at the Multiplex User Meeting. With Leica Biosystems total multiplex solutions and trusted partners, advances in multiplex IHC were innovatively showcased to customers via live presentations, on-demand webinars, virtual exhibition hall, networking room and, virtual lab tour!

This white paper highlights the value of an integrated ISP-in situ hybridization (ISH) workflow for co-detection of protein and RNA targets on a single FFPE tissue section.

Selvam Thavaraj, PhD FDSRCS FRCPath Senior Lecturer in Oral & Maxillofacial Pathology, King’s College London Honorary Consultant in Head & Neck Pathology at Guy’s & St Thomas’ NHS Foundation Image Research interests Biomarkers and the role of microbes in head and neck premalignancy and cancer Diagnostic and prognostic aspects of human papillomavirus in head and neck cancer The webinar will include Method used to conduct the study including study parameters Discussion and results, including agreement with HPV DNA  ISH  and PCR A chance to submit questions for feedback on the topic

Phenotyping Tumor Microenvironment Using DNA Barcoded Multiplex Technology Chifei Sun GSK IVIVTBIBCellular Biomarkers Collegeville, PA I have no conflict of interests with Leica Biosystems, Ultivue and Indica Labs. – All the materials used in this presentation are for educational purpose only. – The human biological samples were sourced ethically, and their research use was in accord with the terms of the informed consents under an IRB/EC approved protocol. Statement 9/25/2020 2 Understand the complexity of tumor microenvironment and its importance in drug development – Introduce the Ultivue DNA barcoding and exchange technology for multiplex immunofluorescence – Highlight the processes of image fusion, spatial analysis and colocalization of markers on individual cells Learnin...

Introduction to immuno oncology 1. Introduction to Immuno-Oncology Michael Bates, MD Oncology Strategy, Medical and Scientific Affairs Cepheid Sunnyvale, CA Cepheid and Leica Biosystems are Danaher Companies 2. Overview • History of Immuno-Oncology • Types of Immunotherapy • Immunomodulatory agents • Personalized Cancer Vaccines • CAR-T cell therapy • Immune Checkpoint Inhibitors • Focus: Immune Checkpoint Biomarkers 3. Evolution of Cancer Immunotherapy • William Coley – 1890s surgeon • Coley’s toxin… • BCG, IFN-alpha, IL-2 • IL-2 applications in melanoma and RCC proved that advanced metastatic cancers can be controlled in some patients by stimulating T cells with cytokines • Critical development • CTLA-4 shown to be inhibitory for T cell responses by James Allison and Jeff Blu...

San Diego, CA – November 8, 2022. Leica Biosystems, a technology leader in automated staining and brightfield and fluorescent imaging, has acquired Cell IDx, Inc., a leader in multiplexed tissue profiling. Founded in 2012 and headquartered in San Diego, California, Cell IDx provides multiplex staining panels, tissue staining, and imaging and analysis services. Cell IDx’s UltraPlex technology allows simultaneous detection of multiple biomarkers in many immunoassays through fluorescent and chromogenic multiplex immunohistochemistry (“IHC”) staining technology, and automated chromogenic detection of multiple markers on Leica Biosystems’ BOND RX fully automated research stainer. Together, UltraPlex and BOND RX with high throughput imaging allows researchers to detect three or more ...