Leica VT1200 - Semiautomatic Vibrating Blade Microtome


Basic instrument with blade holder
without ice tray
without buffer tray, without specimen disc


Instrument features:
Semiautomatic Vibrating Blade Microtome for
sectioning fixed and unfixed specimens from
Neuropathology, Neurophysiology (Patch clamping),
Botany (roots and parts of plants) and Industry
(foams).
Robust and stabile basic instrument with blade
holder for safe insertion of the whole razor blade
and adjustment screw to minimize the vertical
deflection of the blade below 1 µm.
Separate control panel - foil protected. Adjustment
screw for manual feeding prior to each section
(semiautomatic cutting mode).
Support for removable ice tray, double walled buffer
tray or optional measurement device "Vibrocheck",
for vertical deflection of the blade.


Technical specifications:

• Vertical specimen feed: max. 20 mm (depending on selected specimen holder)
• Horizontal stroke: 45 mm
• Blade holder turnable by 90° for safe blade insertion of whole razor-injector or sapphire blades. Clearance angle adjustment of 15°, 18° or 21°. Optimized blade holder design for minimised buffer spillage.
• Separate control panel - foil protected
• Parameter setting for speed, amplitude, section thickness (semiautomatic mode)
• Display for vertical blade deflection value (in µm) and indication of screw turn direction to minimise the vertical blade deflection when using measurement device "Vibrocheck"
• Motorized blade holder vibration with minimal vertical blade deflection (value measurable by optional measurement device "Vibrocheck". Value can be minimised by using adjustment screw on blade holder).
• Motorised blade holder travel speed adjustable between 0.01 - 1.5 mm/s in:
• 0.01 - 0.1 in 0.01 mm/s steps
• 0.1 - 0.5 in 0.02 mm/s steps
• 0.5 - 1.5 in 0.1 mm/s steps
• Accelerated motorised blade return travel speed of 2.5 mm/s
• Manual blade forward and return speed of 1.5 mm/s
• Amplitude adjustment: 0-3 mm in 0.05 mm steps
• Frequency: 85 Hz /- 5 Hz
• "Up" button for fast approach of the specimen to the blade
• "Down" button for fast approach of the home position for safe and fast specimen change
• Adjustment screw for manual feeding prior to each section
• Adjustable step sizes for manual feeding: 1, 10 or 100 µm
• Memo button to save selected section thickness
• Section thickness totalizer, re-settable at any position
• Integrated LED illumination
• Adapter for magnifier or microscope carrier

Technical Data:
Dimensions (LxWxH) without accessories:
600 x 250 x 230 mm
23.62 x 9.8 x 9.0 inches
Dimensions (LxWxH) with magnifier:
600 x 250 x 320 mm
23.62 x 9.8 x 12.59 inches
Dimensions (LxWxH) with microscope:
600 x 250 x 490 mm
23.62 x 9.8 x 19.29 inches
Dimensions (LxWxH) separate control panel:
165 x 120 x 72 mm
6.5 x 4.7 x 2.8 inches

Weight: 65 kg / 143.3 lbs
Admissions: CE, C CSA US
Range of working temperature: 10°C - up to 35°C
Relative humidity: max. 60 %



Standard delivery:
1 Basic instrument (14 0481 42065)
1 Control panel (14 0481 43395)
1 Tool set (14 0481 45999):

• 1 Allen key with handle, size 3.0 (14 0194 04764)
• 1 Allen key with handle, size 6.0 (14 0222 04141)
• 1 Cryo-Manipulator (14 0462 28930)
• 2 Fuses T 1.0A (14 6943 01000) 1 Cyanacrylate glue (14 0371 27414) 1 Dust cover, small (14 0212 43742) 1 IFU-Bundle Leica VT1200 (14 0481 80001) consisting of:
• 1 Instructions for use for Leica VT1200, printed, EN
• 1 CD including available languages
• 1 IFU-language order 1 Short Instruction Manual Leica VT1200 - EN/DE (14 0481 80004)

Leica VT1200 S - Fully Automated Vibrating Blade Microtome


Basic instrument with blade holder
without ice tray
without buffer tray, without specimen disc


Instrument features:
Fully Automated Vibrating Blade Microtome for
sectioning fixed and unfixed specimens from
Neuropathology, Neurophysiology (Patch clamping),
Botany (roots and parts of plants) and Industry
(foams).
Robust and stabile basic instrument with blade
holder for safe insertion of the whole razor blade
and adjustment screw to minimize the vertical
deflection of the blade below 1 µm.
Separate control panel - foil protected. Automatic
specimen retraction during blade return stroke and
automatic specimen feeding prior to each section.
Support for removable ice tray, double walled buffer
tray or optional measurement device "Vibrocheck",
for vertical deflection of the blade.


Technical specifications:

• Vertical specimen feed: max. 20 mm (depending on selected specimen holder)
• Horizontal stroke: 45 mm
• Blade holder turnable by 90° for safe blade insertion of whole razor-injector or sapphire blades. Clearance angle adjustment of 15°, 18° or 21°. Optimized blade holder design for minimised buffer spillage.
• Separate control panel - foil protected
• 8 individually programmable parameter settings
• Parameter setting for speed, amplitude, section thickness (automatic mode)
• Display for vertical blade deflection value (in µm) and indication of screw turn direction to minimise the vertical blade deflection when using measurement device "Vibrocheck"
• Motorized blade holder vibration with minimal vertical blade deflection (value measurable by optional measurement device "Vibrocheck". Value can be minimised by using adjustment screw on blade holder).
• Motorised blade holder travel speed adjustable between 0.01 - 1.5 mm/s in:
• 0.01 - 0.1 in 0.01 mm/s steps
• 0.1 - 0.5 in 0.02 mm/s steps
• 0.5 - 1.5 in 0.1 mm/s steps
• Accelerated motorised blade return travel speed of 2.5 mm/s
• Amplitude adjustment: 0-3 mm in 0.05 mm steps
• Frequency: 85 Hz /- 5 Hz
• Selection between semi-motorized or fully automatic cutting mode
• Freely programmable cutting window, two individualy programmable cutting window edges
• Two fully motorized cutting modes: Single or continuous mode
• Specimen retraction during blade holder return stroke adjustable between 0 and 100 µm steps
• Manual blade forward and return speed adjustable between 1-5 mm/s in 0.5 mm/s steps.
• Selection between vibration on or off during manual blade forward or return movement to approach specimen
• "Up" button for fast approach of the specimen to the blade
• "Down" button for fast approach of the home position for safe and fast specimen change
• Adjustment screw for manual feeding prior to each section (if using semiautomatic mode)
• Adjustable step sizes for manual feeding: 1, 10 or 100 µm
• Memo button to save selected section thickness
• Section thickness totalizer, re-settable at any position
• Integrated LED illumination, individually programmable in 5 steps
• Adapter for magnifier or microscope carrier

Technical Data:
Dimensions (LxWxH) without accessories:
600 x 250 x 230 mm
23.62 x 9.8 x 9.0 inches
Dimensions (LxWxH) with magnifier:
600 x 250 x 320 mm
23.62 x 9.8 x 12.59 inches
Dimensions (LxWxH) with microscope:
600 x 250 x 490 mm
23.62 x 9.8 x 19.29 inches
Dimensions (LxWxH) separate control panel:
165 x 120 x 72 mm
6.5 x 4.7 x 2.8 inches

Weight: 65 kg / 143.3 lbs
Admissions: CE, C CSA US
Range of working temperature: -10°C - up to 35°C
Relative humidity: max. 60 %



Standard delivery:
1 Basic instrument (14 0481 42066)
1 Control panel (14 0481 43396)
1 Tool set (14 0481 45999):

• 1 Allen key with handle, size 3.0 (14 0194 04764)
• 1 Allen key with handle, size 6.0 (14 0222 04141)
• 1 Cryo-Manipulator (14 0462 28930)
• 2 Fuses T 1.0A (14 6943 01000) 1 Cyanacrylate glue (14 0371 27414) 1 Dust cover, small (14 0212 43742) 1 EC Declaration of Conformity (14 0481 80011) 1 IFU-Bundle Leica VT1200 S (14 0481 80001) consisting of:
• 1 Instructions for use for Leica VT1200 S, printed, EN
• 1 CD including available languages
• 1 IFU-language order 1 Short Manual Leica VT1200 S - EN/DE (140481 80005)

Leica VT1200 - Configuration

Leica VT1200 - Configuration
with blade holder, ice tray, buffer tray - metal,
and specimen disc


Instrument features:
Semiautomatic Vibrating Blade Microtome for
sectioning fixed and unfixed specimens from
Neuropathology, Neurophysiology (Patch clamping),
Botany (roots and parts of plants) and Industry
(foams).
Robust and stabile basic instrument with blade
holder for safe insertion of the whole razor blade
and adjustment screw to minimize the vertical
deflection of the blade below 1 µm.
Separate control panel - foil protected. Adjustment
screw for manual feeding prior to each section
(semiautomatic cutting mode).
Support for removable ice tray, double walled buffer
tray or optional measurement device "Vibrocheck",
for vertical deflection of the blade.


Technical specifications:

• Vertical specimen feed: max. 20 mm (depending on selected specimen holder)
• Horizontal stroke: 45 mm
• Blade holder turnable by 90° for safe blade insertion of whole razor-injector or sapphire blades. Clearance angle adjustment of 15°, 18° or 21°. Optimized blade holder design for minimised buffer spillage.
• Separate control panel - foil protected
• Parameter setting for speed, amplitude, section thickness (semiautomatic mode)
• Display for vertical blade deflection value (in µm) and indication of screw turn direction to minimise the vertical blade deflection when using measurement device "Vibrocheck"
• Motorized blade holder vibration with minimal vertical blade deflection (value measurable by optional measurement device "Vibrocheck". Value can be minimised by using adjustment screw on blade holder).
• Motorised blade holder travel speed adjustable between 0.01 - 1.5 mm/s in:
• 0.01 - 0.1 in 0.01 mm/s steps
• 0.1 - 0.5 in 0.02 mm/s steps
• 0.5 - 1.5 in 0.1 mm/s steps
• Accelerated motorised blade return travel speed of 2.5 mm/s
• Manual blade forward and return speed of 1.5 mm/s
• Amplitude adjustment: 0-3 mm in 0.05 mm steps
• Frequency: 85 Hz /- 5 Hz
• "Up" button for fast approach of the specimen to the blade
• "Down" button for fast approach of the home position for safe and fast specimen change
• Adjustment screw for manual feeding prior to each section
• Adjustable step sizes for manual feeding: 1, 10 or 100 µm
• Memo button to save selected section thickness
• Section thickness totalizer, re-settable at any position
• Integrated LED illumination
• Adapter for magnifier or microscope carrier
• Removable ice tray, drawer principle, allows preparation of the tissue under the microscope with integrated palm rests with magnets to hold buffer tray in place
• Buffer tray (plastics) with lid to cover up buffer tray while filling ice tray, magnet to hold specimen disc in place, specimen disc - non-orienting and clamp to hold tube in buffer during oxygenation. Volume: 125 ml, autoclavable

Technical Data:
Dimensions (LxWxH) without accessories:
600 x 250 x 230 mm
23.62 x 9.8 x 9.0 inches
Dimensions (LxWxH) with magnifier:
600 x 250 x 320 mm
23.62 x 9.8 x 12.59 inches
Dimensions (LxWxH) with microscope:
600 x 250 x 490 mm
23.62 x 9.8 x 19.29 inches
Dimensions (LxWxH) separate control panel:
165 x 120 x 72 mm
6.5 x 4.7 x 2.8 inches

Weight: 65 kg / 143.3 lbs
Admissions: CE, C CSA US
Range of working temperature: 10°C - up to 35°C
Relative humidity: max. 60 %



Standard delivery:
1 Basic instrument (14 0481 42065)
1 Control panel (14 0481 43395)
1 Tool set (14 0481 45999):

• 1 Allen key with handle, size 3.0 (14 0194 04764)
• 1 Allen key with handle, size 6.0 (14 0222 04141)
• 1 Cryo-Manipulator (14 0462 28930)
• 2 Fuses T 1.0A (14 6943 01000) 1 Cyanacrylate glue (14 0371 27414) 1 Dust cover, small (14 0212 43742) 1 IFU-Bundle Leica VT1200 (14 0481 80001) consisting of:
• 1 Gebrauchsanweisung Leica VT1200, gedruckt, EN
• 1 CD mit verfügbaren Sprachen
• 1 IFU-language order 1 Short Manual VT1200 - EN/DE (14 0481 80004) 1 Ice tray, assy. (14 0481 42010) 1 Buffer tray, metal (14 0481 42084) consisting of:
• 1 Buffer tray
• 1 Lid
• 1 Specimen disc, non-orienting
• 1 Tube clamp 1 Blade holder (attached to the instrument)

Leica VT1200 S - Configuration

Leica VT1200 S - Configuration
with blade holder, ice tray, buffer tray - metal,
and specimen disc


Instrument features:
Fully automated Vibrating Blade Microtome for
sectioning fixed and unfixed specimens from
Neuropathology, Neurophysiology (Patch clamping),
Botany (roots and parts of plants) and Industry
(foams).
Robust and stabile basic instrument with blade
holder for safe insertion of the whole razor blade
and adjustment screw to minimize the vertical
deflection of the blade below 1 µm.
Separate control panel - foil protected. Adjustment
screw for manual feeding prior to each section
(semiautomatic cutting mode).
Support for removable ice tray, double walled buffer
tray or optional measurement device "Vibrocheck",
for vertical deflection of the blade.


Technical specifications:

• Vertical specimen feed: max. 20 mm (depending on selected specimen holder)
• Horizontal stroke: 45 mm
• Blade holder turnable by 90° for safe blade insertion of whole razor-injector or sapphire blades. Clearance angle adjustment of 15°, 18° or 21°. Optimized blade holder design for minimised buffer spillage.
• Separate control panel - foil protected
• Parameter setting for speed, amplitude, section thickness (semiautomatic mode)
• Display for vertical blade deflection value (in µm) and indication of screw turn direction to minimise the vertical blade deflection when using measurement device "Vibrocheck"
• Motorized blade holder vibration with minimal vertical blade deflection (value measurable by optional measurement device "Vibrocheck". Value can be minimised by using adjustment screw on blade holder).
• Motorised blade holder travel speed adjustable between 0.01 - 1.5 mm/s in:
• 0.01 - 0.1 in 0.01 mm/s steps
• 0.1 - 0.5 in 0.02 mm/s steps
• 0.5 - 1.5 in 0.1 mm/s steps
• Accelerated motorised blade return travel speed of 2.5 mm/s
• Amplitude adjustment between 0-3 mm in 0.05 mm steps
• Frequency: 85 Hz /- 5 Hz
• Selection between semi-motorized or fully automatic cutting mode
• Freely programmable cutting window, two individually programmable cutting window edges
• Two fully motorised cutting modes. Single or continuous mode
• Specimen retraction during blade holder return stroke adjustable between 0 and 100 µm in 10 µm steps
• Manual blade forward and return speed adjustable between 1-5 mm/s in 0.5 mm/s steps
• Selection between vibration on or off during manual blade forward or return movement to approach specimen
• "Up" button for fast approach of the specimen to the blade
• "Down" button for fast approach of the home position for safe and fast specimen change
• Adjustment screw for manual feeding prior to each section (if using semiautomatic mode)
• Adjustable step sizes for manual feeding: 1, 10 or 100 µm
• Section thickness totalizer, re-settable at any position
• Integrated LED illumination, individually programmable in 5 steps
• Adapter for magnifier or microscope carrier
• Removable ice tray, drawer principle, allows preparation of the tissue under the microscope with integrated palm rests with magnets to hold buffer tray in place
• Buffer tray (plastics) with lid to cover up buffer tray while filling ice tray, magnet to hold specimen disc in place, specimen disc - non-orienting and clamp to hold tube in buffer during oxygenation. Volume: 125 ml, autoclavable

Technical Data:
Dimensions (LxWxH) without accessories:
600 x 250 x 230 mm
23.62 x 9.8 x 9.0 inches
Dimensions (LxWxH) with magnifier:
600 x 250 x 320 mm
23.62 x 9.8 x 12.50 inches
Dimensions (LxWxH) with microscope:
600 x 250 x 490 mm
23.62 x 9.8 x 19.29 inches
Dimensions (LxWxH) separate control panel:
190 x 150 x 72 mm
7.48 x 5.90 x 2.83 inches

Weight: 65 kg / 143.3 lbs
Admissions: CE, C CSA US
Range of working temperature: 10°C - up to 35°C
Relative humidity: max. 60 %



Standard delivery:
1 Basic instrument (14 0481 42066)
1 Control panel (14 0481 43396)
1 Tool set (14 0481 45999):

• 1 Allen key with handle, size 3.0 (14 0194 04764)
• 1 Allen key with handle, size 6.0 (14 0222 04141)
• 1 Cryo-Manipulator (14 0462 28930)
• 2 Fuses T 1.0A (14 6943 01000) 1 Cyanacrylate glue (14 0371 27414) 1 Dust cover, small (14 0212 43742) 1 Ice tray, assy. (14 0481 42010) 1 Buffer tray, metal (14 0481 42084)consisting of:
• 1 Buffer tray
• 1 Lid
• 1 Specimen disc, non-orienting
• 1 Tube clamp 1 Blade holder (attached to the instrument) 1 IFU-Bundle Leica VT1200 S (14 0481 80001) constisting of:
• 1 Instructions for use Leica VT1200 S, printed, EN
• 1 CD including available languages
• 1 IFU-langage order 1 Short Manual Leica VT1200 S - DE/EN (140481 80005)

Blade holder, assy.

Sapphire knife vor VT1000 S / VT1200 / S

Knife angle: 22 degrees
Material blade: Synthetic Single Crystal Sapphire
Height blade: 12 mm/ 0.5 inches
Thickness edge: 0.9 mm/ 0.035 inches
Length: 40 mm/ 1.5 inches

Antifrogen N - heat transfer liquid, 10 l
for use with Julabo FL300 Chiller

Note: must be diluted 50:50 with water before
use with Julabo FL300 Chiller

Cyanoacrylate adhesive for mounting
specimens onto specimen discs, 10 g

Buffer tray, plastic - consisting of:

• Buffer tray
• Lid
• Specimen disc, non-orienting (140481 41969)
• Tube clamp

Volume: 125 ml, autoclavable

Buffer tray, double-walled to be used with
Julabo FL300 chiller.

consisting of:

1 buffer tray, double-walled
1 specimen disc, non-orienting (140481 41969)
2 tube clamps
2 silicone hoses, 1.5 m long,
6 mm diameter, with isolation
1 hose coupling, assy.


volume: 400 ml, can not be autoclaved
inner dimension: 12.6 mm

Buffer tray, metal - consisting of:

• Buffer tray
• Lid
• Specimen disc, non-orienting (140481 42086)
• Tube clamp

Volume: 125 ml, can not be autoclaved

Julabo - FL300 Recirculating Chiller - 115 V/60 Hz


Recirculating Chiller for environmentally friendly
cooling.
The FL series offers a new generation of chillers
for routine cooling applications within the laboratory
and industry. The working temperature range covers -20°C
to 40°C, the temperature stability of the
PID control is ±0.5°C. All units can easily be cleaned
and are provided with a splash water proof keypad
with LED temperature indication. On the front of the
units there is an RS232 interface as well as an
alarm shutdown.
The filling port is easily accessible placed on
the top under a lift-up cover. Another hinged tray
serves as a file for the operating manual or other
documents concerning the installation. The removible
venting grid allows an easy cleaning of the condenser,
the drain tap is easily acceable behind the grid.
All models include an easily visible level indication.
Another advantage is the venting slots are on the
front and rear and therefore the units can be placed
directly one beside the other (space saving).



Advantages

• Ergonomic design and easy operation
• Splash-proof keypad with integrated mains switch
• Large, bright LED display
• Reliable Microprocessor PID temperature control
• Filling level indicator
• Powerful immersion pumps, suitable for continuous operation
• Permissible temperature in return line 80°C
• Easy filling from the top with hinged protective lid
• Low liquid level protection with optical and audible alarm signal
• Integrated stainless steel bath tanks
• Removable venting grid for cleaning of the condenser
• Front drain
• No side vents
• RS232 interface for PC-connection
• IP class according to IEC 60529: 21

Technical Specifications:

Working temperature range -20 - 40 °C
Temperature control PID
Temperature stability ±0.5 °C
Display LED
Display resolution 0.1 °C
Integrated programmer not available
Heater capacity 0 W
Cooling capacity
Refrigerant R134a
Pump capacity Pressure: 0.35 bar
Flow rate: 15 l/min
Digital interfaces RS232
Filling volume 3.5 - 4 Liters
Dimensions (W x L x H) 25 x 50 x 60 cm
Weight 39 kg
Ambient temperature 5 - 40 °C
Classification according to DIN 12876-1 -



Miscellaneous

Included: 2 each barbed fittings for tubing
8 and 12 mm inner dia. (pump connections M16x1 male)
Suitable fluids: water, water-glycol mixture,
JULABO Thermal bath fluids

Cooling hose (pack of 2) - inlcluding hose couplings.
length: 1.50 m

Cooling hose is included in standard delivery
of VT1000 S and VT1200/s double-walled buffer trays.

Clamp to hold tube in buffer during oxygenation.
To be used with buffer trays (0481 42089,
0481 42084 and 0481 44837)

Specimen disc, aluminium, painted/ powdercoated black

• non-orienting
• For specimens up to 20 mm
• 360° rotatable
• Kept in buffer tray by magnet

Specimen disc, aluminium, painted/powdercotaed black

• non-orienting
• for specimens up to 10 mm

Specimen disc, orienting
With 2.5 ° and 5 ° indicators for reproducible
specimen tilting

Vibrocheck - measurement device for vertical
deflection of the blade

Microscope consisting of:

• 1 Microscope carrier
• 1 Microscope Leica S6 Stereozoom
• 2 Oculars 10 x 23 B, adjustable
• 1 Objective protecting glass
• 1 Dust cover, large
• 1 Cover LED socket
• 1 Adapter for 2-arms LED Hi-Power spots

Cover LED socket

Magnifier with carrier, 2-fold magnification
consisting of:

• Lid
• Dust cover, large
• Adapter for 2-arms LED Hi-Power spots

Lid for buffer tray
(140481 42089 and 140481 42084)

Protective cover, small

Protective cover, large
To be used with microscope or magnifier

LED Hi-Power spots, 2-arm
100-240 V

NOTE:
This module can only function with Hi-Power Spot
LED 1000 - control unit (14600004825)

Hi-Power Spot, LED 1000
Standard control unit including power cord with 4 adapters
100-240 V, 50-60 Hz

NOTE:
This module can only function with LED Hi-Power Spots,
2-arm (14600004826)

Foot switch for Leica VT1200 / S