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Fundamentals of Hematoxylin and Eosin Staining

Cindy Sampias, JD CT(ASCP)HTL

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For characterizing tissue, the use of H&E is by far preferred for viewing cellular and tissue structure detail. The variation of stain intensity is often driven by the slide reviewers' learning experience and what they are comfortable seeing. Because this stain demonstrates a broad range of cytoplasmic, nuclear, and extracellular matrix features, nearly all teaching texts use H&E images. We continue to use this simple and essential stain today, which has remained unchanged for over a century.

Learning Objectives

  • Identify the four components of an H&E stain
  • Describe the critical elements of developing an H&E staining protocol
  • Define three characteristics of an optimal and suboptimal H&E stain

In this webinar, Cindy Sampias, JD CT(ASCP)HTL, Applications Technical Team Lead - Core Histology at Leica Biosystems, provides an overview and discusses the practical uses of the Hematoxylin and Eosin stains gleaned from over 30 years of laboratory experience in the field of histology.

During this presentation, Cindy provides insights and shares fundamental concepts about H&E staining, including the components, types, and “how-to” elements of developing protocols. Cindy shares “tips and tricks” with the audience on performing H&E staining optimizations and concludes by describing best practice examples of what to look for in optimal and suboptimal H&E stains.

For Research Use Only. Not for Use in Diagnostic Procedures.

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About the presenter

Cindy Sampias, JD CT(ASCP)HTL

Cindy Sampias is a board certified Cyto- and Histo-technologist and one of the original Core Histology Applications Specialists at Leica Biosystems. She is currently a Senior Field Applications Specialist at Leica Biosystems. With more than 25 years of experience, she is a guest speaker at histology and cytology meetings around the country. She is a technical author for Media Lab, publishing a variety of technical courses and sharing best practices in histology.