Aperio GT 450 DX System

81 slides per hour @ 40x (15mm x 15mm area).

32 seconds at 40x for 15mm x 15mm area

No, Aperio GT 450 DX has a scalable IT architecture solution …where a centralised SAM server is used to set up and monitor multiple GT 450 DX scanners from the IT professionals' office.

Scanner Administration Manager DX (SAM) is a high-performance server installed in the IT room that enables a centralized admin hub that is connected to multiple GT 450 DX scanners in the pathology lab. The GT 450 DX scanners stream raw image files to the SAM server after which they are converted to an SVS file. SAM includes Mirth for data and event handling and HL7 for integration. SAM DX server client application software is loaded on the SAM DX server and enables IT professionals and lab directors to log in via secure network access and then set up and monitor GT 450 DX scanners in the lab, including dashboard status views, PIN controls for each scanner and log out times for each scanner.

Yes, in the rare case you need to adjust the tissue finder, the user can simply select “Scan Entire Slide” and the Aperio GT 450 DX will automatically scan the entire slide.

No, this is automated with the Aperio GT 450 DX because it uses Real-Time Focusing.

Yes, Aperio GT 450 DX images are designed to be viewed on calibrated monitors for the best possible viewing experience as it relates especially to color. The Aperio Viewer DX Stations, include monitors that are calibrated and haven been validated for use with the GT 450 DX images. The Aperio viewing station includes a proprietary image color correction (ICC) profile on the workstation that calibrates the monitor for use with GT 450 DX next generation images.

Yes, during each scan, the scanning workflow includes a system calibration. This calibration is included in the 32 sec scan speed at 40x for a 15mm X 15mm area.

0.26 µm/pixel

Yes. This unique open face design allows up to 14 additional racks to be added or removed while scanning is in progress.

Yes, these racks can be used across 3 of our devices, including Aperio GT 450 DX, HistoCore SPECTRA workstation ST Stainer, and HistoCore SPECTRA workstation CV Coverslipper.

DICOM (Digital Imaging and Communications in Medicine) is a standard for medical image interchange that includes support for transmission and storage of medical image data.

It can be used to transfer data from a Picture Archiving and Communication System (PACS) into some other system, produced by any number of vendors who choose to implement that standard, or used to provide access to clinical data without granting that person access to the entire PACS, or to share medical image data with a patient or colleague, or for long-term archival purposes.

DICOM is an open, vendor-agnostic standard for medical image data. While many vendors participate in the creation of this standard, it is not controlled by any one vendor. As an open standard, it may be implemented by any vendor and enables interoperability between systems.

The DICOM standard provides a definition for Whole Slide Microscopy Images, commonly referred to as a “Supplement 145 image” (after the DICOM Supplement which initially defined the image) or “Working Group 26 image” (after the DICOM Working Group which produced Supplement 145). As this standard is intended to be usable by manufacturers of both tiling and line scanning systems, as well as different modalities (e.g. oil, fluorescence, brightfield), it provides vendors multiple ways to produce images that are conformant to the standard, but may not necessarily be compatible with a given vendor’s PACS or viewing software.

The Aperio GT 450 DX outputs images as a series of Whole Slide Microscopy Image objects. As the Aperio GT 450 DX scanner is intended to operate as a networked device, it does so by outputting the images using standard DICOM network transfer protocols. The Aperio GT 450 DX scanner can transfer images directly into a customer PACS, which they can then export from their PACS to access the DICOM files.

If you are using a PACS capable of receiving and storing Whole Slide Microscopy Images and a viewer capable of presenting the images, then yes. However, not all PACS vendors support Whole Slide Microscopy Images, and due to the size of these images, not all PACS systems that do will do so with acceptable performance.

Similarly, due to the size of the images not all DICOM viewers will be capable of presenting the images correctly or with good performance. And, as with Aperio SVS file format, the Whole Slide Microscopy Images generated by the Aperio GT 450 DX product family consist of thousands of smaller image “tiles,” and not all DICOM viewers are capable of handling this type of image.

Yes, Leica Biosystems Aperio DICOM Conformance Statement (MAN-0465) is intended to facilitate integration between the Aperio GT 450 DX product family scanners and other DICOM products. It includes detailed information about how the Aperio GT 450 DX produces DICOM files, DICOM functionality, and how that functionality integrates with other devices that support DICOM features.

If you are interested in using DICOM images from the Aperio GT 450 DX product family, please contact your local Leica Biosystems sales representative. We will work with you to understand your organization’s needs, and how Aperio GT 450 DX DICOM can integrate into your PACS workflow.

The Aperio GT 450 DX scans a standard 15mm x 15mm tissue area at 40x magnification in 32 seconds per slide, achieving an operational throughput of 81 slides per hour (validated under Leica Biosystems protocol VP-0450). It features a 450-slide capacity across 15 continuous-loading racks.

Covered by US Patent No. 9,841,590, Real-Time Focus utilizes dual sensors—an imaging line sensor and a dedicated focusing line sensor. While scanning, the focusing sensor captures real-time surface profile data of the tissue. Proprietary algorithms adjust the objective lens height on the fly in a continuous feedback loop, maintaining sharp focal accuracy at 40x magnification without stopping to map focal points.

Yes. The Aperio GT 450 DX supports native DICOM-compatible image output alongside Leica’s SVS and TIFF file formats. This allows direct integration into enterprise digital pathology systems, vendor-neutral archives (VNAs), PACS, and Laboratory Information Systems (LIS) via standard HL7 interfaces and Mirth Connect logging.

The GT 450 DX accepts Leica Universal Racks (30-slide capacity) loaded directly from the HistoCore SPECTRA Stainer and Coverslipper Workstation without manually handling individual glass slides. It is also compatible with Sakura Prisma Stainer/Coverslipper racks (20-slide capacity).

Optional Aperio iQC DX software uses AI-enabled algorithms to evaluate whole slide images (WSIs) automatically during acquisition. It detects visual artifacts, glass defects, or out-of-focus areas, alerting histotechnicians through a centralized dashboard and suggesting corrective actions before slides are routed to pathologists.

The system requires a dedicated Scanner Administrator Manager (SAM) DX server. Network bandwidth guidelines require 1 Gigabits per second (1 Gbps) connection from individual GT 450 DX units to the SAM Server, and a 10 Gigabits per second (10 Gbps) pipe from the SAM Server to the central image repository or PACS.

The scanner accommodates standard 2.54 cm x 7.62 cm 1 inch x 3 inch) slides complying with ISO 8037/1 standards, with glass thickness between 0.9 mm and 1.1 mm. It supports standard glass coverslips (0.17 mm thickness) with Eukitt mounting media or Cellulose Tri-Acetate film coverslips. Tissue specimen thickness is optimized for 3–5µm.

In laboratories handling over 120,000 slides annually, the GT 450 DX increases productivity through continuous loading, reducing technician manual handling time. Features like 99.5% tissue finder accuracy and 100% barcode recognition (VP-0463) minimize manual intervention, while AI quality control reduces repeat slide scanning expenses.