The localization of Nerve Cell Antigens in rat brain with the Leica VT1000 vibrating blade microtome

Immunohistochemistry is an important research method to study the central nervous system (CNS). During the last three decades, different immunodetection systems have been developed.

CNS antigens can be detected by isotopic, enzymatic and fluorescence systems. Although these methods can be used on brain sections obtained with a cryostat, more accurate localization and superior morphological detail requires the use of non-frozen tissue.

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