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Immunofluorescence – Fluorescent Immunohistochemistry Image Analysis

The Aperio Image Analysis Immunofluorescence menu offers solutions for measurement of single and multiplex tissue staining. Quantify expression of fluorescently-labeled protein biomarkers at the tissue, cellular or subcellular level. Algorithms can be customized for a variety of fluorescent dyes, allowing each algorithm to be used for specific, diverse research requirements.

Aperio RUO (Research Use Only) Image Analysis Algorithms have been validated by Leica Biosystems for use with .svs images from Aperio AT2, Aperio CS2, and Aperio VERSA scanners. Use of Aperio RUO Algorithms with other available scanners has not been validated, and Leica Biosystems cannot train or support customers in use of Aperio RUO Algorithms with images from these scanners.

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Aperio Cellular IF Algorithm

Aperio Cellular IF Algorithm

Quantify location, area, intensity, and colocalization up to 7 FL-labeled biomarkers within membrane, nuclear, and cytoplasmic cellular compartments.

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Aperio Area Quantification FL Algorithm

Identify and measure multiple fluorescent dyes in tissue, including area and intensity of each dye, and channel colocalization.

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Aperio Positive Pixel Count FL Algorithm

Accurate, easy-to-use measurement of biomarker expression based on fluorescent dye signal.

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New and relevant information, ranging from the basics to specific application know-how.

Introduction to Immunofluorescence
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