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The Power of Spatially Resolved Staining

Bradley Spencer-Dene, Subject Matter Expert, Next Gen Histology at GSK
Elena Miranda, PhD, Director, Non-Clinical Histology at GSK

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Stay up-to-date with the latest research studies in spatially resolved staining techniques.

Chromogenic Co-detection of Protein and mRNA Targets in Tissues using the BOND RX Fully Automated Research Stainer

Bradley Spencer-Dene

Subject Matter Expert, Next Gen Histology at GSK

Co-detection of more than one target in Formalin-Fixed Paraffin Embedded samples is an extremely useful application and one that is increasingly required in modern histopathology labs. In order to detect both protein and mRNA targets in the same section we have established a semi-automated workflow for sequentially detecting protein and mRNA targets chromogenically on the BOND RX research stainer combining AP/Fast Red detection (RNAscope/Basescope in situ hybridization) with HRP/Green detection (Immunohistochemistry) to establish target mRNA expression in the context of cell lineage detection.

 

Digital Histopathology Deployment in GSK Non-Clinical Histology Facility

Elena Miranda, PhD

Director, Non-Clinical Histology at GSK

Currently, the Pathology community examines relatively small numbers of digital images of H&E stained histological sections for internal consultation and peer review harmonization. In the Non-Clinical Histology team, we focus on a new digital histopathology imaging workflow that will define the standards for histopathology in drug discovery/development for the wider GSK scientific community and expectations for external collaborators. By consolidating metadata standards, governance and data entry systems, the digital histopathology imaging workflow will enable increased application of quantitative image analytic approaches like artificial intelligence to clinical and nonclinical biomarker assays.

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