Staining
The staining process highlights tissue morphology, cell types, and structure to enable accurate tumor classification and diagnosis. View resources on how staining is practiced today, and how it may evolve in the future.
Technical Brief of Heat Induced Epitope Retrieval
Russell Myers, Ph.D.
The value or power of IHC frequently is compromised by tissue fixation and processing.
Introduction to Immunofluorescence
James Burchette, HT (ASCP)
A review of fluorochromes and the specialized microscope used in immunofluorescence techniques will begin this presentation. A typical (human skin and kidney) clinical specimen will be followed from receipt in the laboratory, through freezing, cryomicrotomy...
The Impact of Decalcification on Staining
Loralee McMahon, MS HTL (ASCP)
Different types of cancers frequently metastase to bone tissue. Treatment planning decisions are often based upon histology and special staining of these distant sites of disease. These decisions may rely on the outcome of immunohistochemistry, in situ...
Factor XIIIa Expression in Normal and Pathological Skin
Guy Orchard, Ph.D.
Catherine Stefanato, Ph.D.
Techniques to Minimize Articular Cartilage Folding
Sarah A. Mack
After a joint has been decalcified, the loss of proteoglycans in the articular cartilage causes it to fold and ripple during the staining process. When this occurs, it makes it impossible to perform histo-morphometry or measurements of the cartilage.
Basic Molecular Pathology and Pre-Analytical Variables
Cecilia Yeung, Pathologist – Seattle Cancer Care Alliance, Assistant Professor – University of Washington, Dept. of Pathology
In this session, we will briefly review the basics of molecular biology, examine critical factors which affect the quality of nucleic acids in the tissues and cells which are submitted for downstream molecular diagnostics, and briefly introduce some...
Understanding How Clinical HPV Testing Leads to Personalized Medicine At Its Best
Human papillomaviruses (HPVs) are the etiologic agents for a significant fraction of many cancers in humans. Our understanding of the pathogenesis of these tumors has led to almost continuous refinement in our approach to managing patients at risk for these...
Single Cells Shine New Light on Lung Biology and Disease
Keith Wharton, MD PhD FCAP
In this webinar, Dr. Keith Wharton reviews a set of published studies using single cell analysis of lung tissues that have provided ground breaking insight into lung development and disease.
Staining ‘Other’ Specimen
Loralee McMahon, MS HTL (ASCP)
In surgical pathology more than ever, the laboratories are expected to do more with less. Taking on more specimens with less technical staff, or getting more stains out of tiny biopsies or fine needle aspirations have become the norm. The College of American...