Slide 1 Hello, hello; thank you so much for that introduction. Let's get started. Oh, I'm sorry, I just need to hit this next button here. So my name is Mark Lawson. Thank you for joining us in this webinar. I'm here to present BOND RX tips, tricks, and optimization. It's going to be a user guide from the BOND RX and chromogenic multiplexing in the research application. Slide 2 So, my name is Mark Lawson. I'm an application specialist on the Life Sciences team at Leica Biosystems I provide technical support for the Life Sciences portfolio, including but not limited to the BOND RX, the BOND RXm, and a wide array of reagents. So I've worked in the histology field for about 15 years and in both clinical and research spaces. I started off as a histotechnologist and worked my way up...

Immunohistochemistry (IHC) detects proteins of interest within whole tissue sections, keeping cellular organization intact.

DESCRIPTION: Combination pack of Cell Palette Red AP, Blue HRP and Yellow HRP chromogen substrates (15mL each) SIZE: 15mL of each of three chromogens APPLICATIONS: IHC COMPONENTS: Cell Palette Red AP chromogen (50X) and substrate buffer (1X). Cell Palette Blue HRP chromogen (50X) and substrate buffer (1X). Cell Palette Yellow HRP chromogen (50X) and substrate buffer (1X). STORAGE: Chromogen – store at 4°C Buffer – for maximum shelf-life store at ...

DESCRIPTION: Yellow chromogen for use with HRP enzyme labeled IHC detection systems SIZE: 50mL APPLICATIONS: IHC COMPONENTS: Cell Palette Yellow HRP chromogen (20X) and substrate buffer (1X). STORAGE: Chromogen – store at 4°C Buffer – for maximum shelf-life store at 4°C. Allow buffer to warm to room temperature prior to use. SHELF-LIFE: 12 months at 4°C INSTRUCTIONSFor each 1.0mL chromogen working solution add 20 uL Chromogen to 980 uL Buffer. On...

DESCRIPTION: Purple hematoxylin counterstain for IHC staining SPECIFICATION: This counterstain is intended to be used by laboratory professionals to stain routinely prepared paraffin-embedded tissue specimens (in vitro) to identify nuclei and cytoplasm. SIZE: 50ml CONCENTRATION: Ready to use, liquid APPLICATIONS: IHC, ISH STORAGE: Room temperature SHELF-LIFE: See expiration date on label PRECAUTIONS: For use by laboratory professionals. See MSDS ...

Multiplexing is an important tool for simultaneous detection of multiple markers within a single tissue section. These educational infographics highlight the 5 key reasons to multiplex, as well as the main considerations for choosing chromogenic or immunofluorescent detection.

DESCRIPTION: Chromogenic multiplex IHC kit for BOND RX - human PD-L1, CD8, PanCK SIZE: 10 slides PRODUCT OVERVIEW: Kit for chromogenic multiplex IHC staining of 10 slides plus a single negative control slide on the BOND RX with V7.0 software. 1° Antibody Clone 1° Tag 2° Antibody Chromogen PD-L1 73-10 UT014 Anti-UT014-HRP Leica Blue CD8 EPR10640(2) UT015 Anti-UT015-AP Leica Refine Red PanCK AE1/AE3 UT016 Anti-UT016-HRP Yellow Leica Ref...