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Aperio Area Quantification FL Algorithm Analyze

Aperio Area Quantification FL Algorithm – Tissue Based Immunofluorescence Measurement

Aperio Area Quantification FL Algorithm

The Aperio Area Quantification FL Algorithm separates a fluorescence stained tissue image of up to 3 channels, and quantifies the intensity and area of each fluorescent dye. It also identifies and measures colocalization between the different channels, providing the user with a full and accurate picture of the biomarker expression pattern in the tissue area.


For Research Use Only. Not for use in diagnostic procedures. For specific product indications and more information click here.

Aperio CS2 and Aperio AT Turbo are registered products under the ScanScope® name.

Copyright © 2016 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH. Aperio is a registered trademark of Leica Biosystems Imaging, Inc. in the and other countries.

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Specify dyes precisely

To get the best results for your specific samples, tell the algorithm which dyes were used on the slide that was scanned. Exclude non-specific fluorescence and highly fluorescent artefacts from analysis with user-defined threshold settings.

Customize settings

Get the most from your fluorescence samples, with precise and accurate image analysis. Aperio Area Quantification FL Algorithm offers highly-tunable inputs to get the most relevant data from your slides, including fine-tuning of channel colors, and the ability to select which dyes to colocalize.

Get in-depth results

View results as numeric data, including Person’s Coefficient and Overlap Coefficient values for each pair of dyes, and plot histograms of the analysis results. A variety of mark-up images can be generated, including colocalization masks for different combinations of dyes.

Do more with your data

Results – including mark-up images – are automatically saved to your Aperio eSlide Manager or Aperio Image Analysis Workstation database, stored alongside the relevant slide. Export results data as CSV files, for further manipulation and analysis in 3rd party statistical programs.