Aperio FISH Brk/Fus Algorithm – Automated FISH Break-apart & Fusion Analysis
The Aperio FISH Brk/Fus Algorithm enables highly-specific and sensitive detection of DNA sequence translocations (break-apart and fusion) in whole slide digital pathology images. It provides standardized results, removing the inter- and intra-user variability that is inherent in manual FISH counts. Aperio FISH Brk/Fus Algorithm has been developed for full interoperability with the Aperio digital pathology solutions for researchers.
Developed with you in mind
Aperio FISH Brk/Fus Algorithm is designed to evaluate break-apart and fusion events within target gene sequences. Specifically tailored to meet workflow challenges, such as the occurrence of “beads on a string” signals, you can be confident that the algorithm is ready to meet your needs.
Easy to use
Set-up of the algorithm is performed via an intuitive tuning wizard, so even inexperienced users can quickly and easily create their own custom analysis tool. Visual mark-up is provided at each stage in the process, with live feedback on results as each setting is changed.
Get the most from your samples
Analyze complex patterns of gene sequence translocation precisely and automatically. Up to 7 signals of interest, in addition to nuclear counterstain, can be analyzed on a single slide. Cells are classified into multiple user-defined categories based on number of signals, break-aparts, and fusions.
Customizable inputs allow the algorithm to be used flexibly with a variety of probes and applications. Users can exclude cells that are not of interest, and adjust signal detection to exclude staining artefacts, haze, and hot signals that may affect analysis.