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Aperio Cellular IF Algorithm – Quantitative Cellular Immunofluorescence Analysis

The Aperio Cellular IF Algorithm enables fast, accurate measurement and colocalization of up to 7 fluorescence channels, plus counterstain. It will distinguish between fluorphores localized within membrane, nuclear and/or cytoplasmic cellular compartments, in digital pathology whole slide tissue images. Retrieve objective, reproducible data from multiplex-stained tissue, which is challenging to quantitate by eye.

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Aperio RUO (Research Use Only) Image Analysis Algorithms have been validated by Leica Biosystems for use with .svs images from Aperio AT2, Aperio CS2, and Aperio VERSA scanners. Use of Aperio RUO Algorithms with other available scanners has not been validated, and Leica Biosystems cannot train or support customers in use of Aperio RUO Algorithms with images from these scanners.

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Multiple tissue types and biomarkers

Customize algorithm input parameters quickly and easily to match your biomarkers, dyes and tissue types. Save settings for later re-use, creating a library of ready-to-use analysis tools for your research.

Understand complex staining patterns

Quantify the expression and colocalization of up to 7 fluorescence channels in membrane, nucleus, and cytoplasm. Define up to 10 scoring classes based on intensity and area of each biomarker of interest across cellular compartments, allowing you to examine multiplex staining in depth for greater understanding of biomarker interactions.

Simple, tailored set up

The tuning workflow is user-friendly and intuitive, guiding you through the steps to create your analysis macro. Set your own names for fluorescence channels and scoring categories, and choose custom mark-up colors for easy visual review.

Clear, comprehensive results

Results are returned per analysis region, plus averaged over all analyzed areas, along with visual mark-up showing which cells fall into each class or into multiple classes. Do a deep dive on the data with per-cell results and the ability to update cell results manually if required.

Part of your total Aperio Digital Pathology research solution

The Aperio Cellular IF Algorithm is optimized for use on images created with Aperio VERSA or Ariol (RUO) and can be deployed either on Aperio Image Analysis Workstation or Aperio eSlide Manager depending on your lab’s throughput requirements. Our digital pathology solutions are developed to work seamlessly together, allowing you to get the most out of your research.