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Aperio Area Quantification FL Algorithm – Tissue Based Immunofluorescence Measurement

The Aperio Area Quantification FL Algorithm separates a fluorescence stained tissue image of up to 3 channels, and quantifies the intensity and area of each fluorescent dye. It also identifies and measures colocalization between the different channels, providing the user with a full and accurate picture of the biomarker expression pattern in the tissue area.

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Aperio RUO (Research Use Only) Image Analysis Algorithms have been validated by Leica Biosystems for use with .svs images from Aperio AT2, Aperio CS2, and Aperio VERSA scanners. Use of Aperio RUO Algorithms with other available scanners has not been validated, and Leica Biosystems cannot train or support customers in use of Aperio RUO Algorithms with images from these scanners.

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Specify dyes precisely

To get the best results for your specific samples, tell the algorithm which dyes were used on the slide that was scanned. Exclude non-specific fluorescence and highly fluorescent artefacts from analysis with user-defined threshold settings.

Customize settings

Get the most from your fluorescence samples, with precise and accurate image analysis. Aperio Area Quantification FL Algorithm offers highly-tunable inputs to get the most relevant data from your slides, including fine-tuning of channel colors, and the ability to select which dyes to colocalize.

Get in-depth results

View results as numeric data, including Pearson’s Coefficient and Overlap Coefficient values for each pair of dyes, and plot histograms of the analysis results. A variety of mark-up images can be generated, including colocalization masks for different combinations of dyes.

Do more with your data

Results – including mark-up images – are automatically saved to your Aperio eSlide Manager or Aperio Image Analysis Workstation database, stored alongside the relevant slide. Export results data as CSV files, for further manipulation and analysis in 3rd party statistical programs.

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