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Chromogenic Multiplex Biomarker Profiling of Human FFPE Tissues Using UltraPlex Technology

Cell IDx’s UltraPlex chromogenic multiplex IHC technology allows the chromogenic detection of 3-4 markers simultaneously on a single tissue section using standard industry workflows. The protocol has been developed to run on the BOND RX autostainer using a single antigen retrieval step and is completed in ~4.5 hours. Adding to its broad utility, BOND RX the technology is primary antibody species independent. Images are obtained on all brightfield scanners in 5-15 minutes. This tissue conserving technology provides maximal information on small tissue samples. FFPE tissue-optimized panels for key biomarkers are available and other panels can be developed on request.

Learning Objectives

  • Learn how UltraPlex chromogenic multiplex IHC technology allows detection of multiple cell subtypes co-expressing multiple biomarkers using optimized chromogen combinations.
  • Learn how to expedite your data generation using a simple, fast, streamlined process combining UltraPlex chromogenic multiplex IHC panels with staining on the BOND RX staining platform and Aperio brightfield scanners.
  • Learn how to increase data generation from limited FFPE tissue samples including TMAs using this tissue conserving technology.

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