Slide 1 Welcome! Slide 2 Intro slide Slide 3 It's nice to meet you! Welcome to the Histology Hacks in Cryosectioning webinar. I am Dr. Marla Rivera-Oliver, and I am very excited to share today some tips and tricks I've learned during my ten years of experience working on basic and translational research. I am based in the Dallas, Texas, area of the United States, and in my current role, I support customers from North Texas and Oklahoma. I completed a bachelor's in biology from the Interamerican University of Puerto Rico and a doctoral degree in Neurobiology from the University of Puerto Rico. I specialized in the spinal cord injury field and honed my skills in Cryosectioning, imaging, and in vivo electrophysiology. Then, I moved into a post-doctoral position at the Center for D...

In the webinar Cryosectioning 101 for Research, John Stock provided a comprehensive guide on achieving better cryosectioning outcomes. John went on to answer customer questions at the end of the session, that have been compiled for your viewing here.

X-tra Slides have a positive charged surface which helps to bond tissue sections and cytology preparations. X‐tra slides are used for demanding H&E procedures that require tissue adhesion. Size: 1” x 3” x .04” (unless otherwise noted) Hydrophobic surface with approximately 90 degree contact angle. Recommended uses include special stains, IHC, ISH, cryosectioning, and cytology.

X-tra Clipped Corner Slides are clipped at 45° angles to help reduce glass breakage and fragmentation when loaded into slide printers. These slides have been optimized for use with the Leica and Sakura Finetek printer. Hydrophobic surface with approximately 90 degree contact angle.  Recommended uses include special stains, IHC, ISH, cryosectioning, and cytology.

FSC 22 is a water soluble embedding compound used in frozen sectioning. The compound bonds and encapsulates tissue specimens to the object holder for cryosectioning. It provides excellent sectioning consistency with minimal curling of sections at a working temperature of -20 °C. FSC 22 is available in clear or in light blue for better visualization of small specimens. It is highly recommended for surgical pathology laboratories....

The CryoJane Tape-Transfer System for Cryosectioning makes it easy to produce high-quality frozen sections of difficult tissues. The CryoJane uses adhesive coated slides and adhesive tapes to capture sections instead of using an anti-roll plate or brush. The Leica CryoJane is adaptable to virtually all cryostats on the market. The system achieves frozen sections of paraffin-quality as thin as two microns. The sections are wrinkle-free, uncompress...

*/ This Item has been discontinued Replaced by HistoCore MULTICUT Halbmotorisches Rotationsmikrotom Arbeitskomfort und Wirtschaftlichkeit stehen im Mittelpunkt des Leica RM2245 Rotationsmikrotoms - sowohl in der klinischen Histologie als auch der Histopathologie. Alle Funktionen steuern Sie über ein kleines, platzsparendes Bedienpult, das Sie links oder rechts von sich platzieren können. Sie können mit dem Leica RM2245 mit der konventionellen Methode einer vollen Handraddrehung oder im „Schaukelbetrieb” arbeiten. Im Schaukelbetrieb wird das Handrad lediglich eine kurze Strecke vor- und zurückgedreht.

*/ This Item has been discontinued Replaced by Leica RM2125 RTS Manuelles Rotationsmikrotom für die klinische Routine-Histologie Das Leica RM2125 haben wir speziell auf die Bedürfnisse und Abläufe des Routinelabors ausgerichtet. Moderne Technik, die sich auf das Wesentliche konzentriert – ohne dabei Kompromisse in Bezug auf Schneidequalität, Bedienkomfort und Anwendersicherheit zu machen. Die ergonomische und kompakte Bauweise des Gerätes sorgt dafür, dass Sie entspannt und konzentriert arbeiten können. Für alle Routine- und Spezialanwendungen und für fast jede individuelle Konfiguration bieten wir Ihnen ein vielfältiges Zuberhörprogramm an.

A review of fluorochromes and the specialized microscope used in immunofluorescence techniques will begin this presentation. A typical (human skin and kidney) clinical specimen will be followed from receipt in the laboratory, through freezing, cryomicrotomy...