Automated Multiplex Immunofluorescence

Bethany Remeniuk, PhD, Global Applications Scientist, Akoya Biosciences, Inc.
Traci DeGeer, BS, HT (ASCP) HTL, QIHC, Director, Advanced Staining Innovation, Leica Biosystems

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Automated IHC Workflow, Multiplex, Immunofluorescence
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Cancer research continues to push the boundaries with new advancements in tissue analysis and biomarker detection. Now, more than ever, there is significant emphasis on understanding the underlying interaction between the immune system and the tumor microenvironment. Multiplexing immunofluorescence (mIF) has greatly increased our understanding of solid tumor biology and immunology, including tumor-infiltrating lymphocytes and cancer-induced architectural alterations, and aided in novel immunology discoveries. In this webinar, we will discuss how Akoya Biosciences Phenoptics assays support quantitative mIF to overcome the limitations imposed by conventional IHC methodologies. We will also discuss how our Opal assay kits and reagents can be integrated with the Leica BOND RX to automate your staining workflow to support consistent results for high-throughput studies.

Learning Objectives

  • Understand the limitations of conventional  IHC , and the benefits of transitioning to multiplex immunofluorescence
  • Learn how to design, optimize and analyze your multiplex assay panel
  • Understand how the BOND RX enables innovations like the Opal assay

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About the presenters

Bethany Remeniuk, PhD, Global Applications Scientist, Akoya Biosciences, Inc.

Bethany Remeniuk is a Research Scientist with extensive working knowledge and diverse skillsets in the fields of cancer biology, medical pharmacology, and neurobiology. Accomplished in the development, design, and execution of both clinical and preclinical studies with successful history of publications. Able to effectively collaborate across different research specialties, both in academia and industry, to achieve work objectives

Traci DeGeer, BS, HT (ASCP) HTL, QIHC, Director, Advanced Staining Innovation, Leica Biosystems

Traci DeGeer is the Director, Advanced Staining Innovation, Leica Biosystems. In this capacity she helps access new technologies for the Life Science research business, manages relationships with partners, works with legal partners to put agreements in place and liaises with Business Units to meet partner/customer needs as technologies are being developed. Traci holds a Bachelor of Science, in Biology, an HT, HTL, and QIHC for the anatomic pathology lab and recently graduated the HBx core program. Traci also holds a patent in small molecule detection for PDL-1 and has spoken at over one hundred state, regional and global symposia on various topics. Traci also sits on the ASCP Board of Certification (HT, HTL and QIHC Exam) and is the current Education Chair for the National Society of Histotechnology.