Automated Multiplex Immunofluorescence
In this webinar, we will discuss how Akoya Biosciences Phenoptics assays support quantitative mIF to overcome the limitations imposed by conventional IHC methodologies. We will also discuss how our Opal assay kits and reagents can be integrated with the Leica BOND RX to automate your staining workflow to support consistent results for high-throughput studies.
Multiplex Immunofluorescence Profiling of Tumor Biopsies Using UltraPlex mxIF Technology
An unmet need in tissue diagnostics is the ability to simultaneously detect three or more markers on a single tissue specimen, i.e. multiplexing. Unlike other immunoassays, i.e. flow cytometry, ELISA and bead assays, which solely quantify the biomarker in a sample, a multiplex immunoassay quantifies the biomarker, identifies the location of the cell in the tissue and identifies the spatial relationship of the biomarkers with respect to each other.
Multiplex fluorescence immunohistochemistry using the Ultivue InSituPlex platform on the Leica Biosystems BOND RX
Multiplex fluorescence immunohistochemistry offers a window into the biology of human disease, enabling the analysis of target protein expression in subsets of specific cells within the context of histopathological features of disease.
Design, optimization, and validation of multiplex immunofluorescence panels
In this webinar, we will cover biological and technical considerations when designing a panel, a linear process for developing, testing, and optimizing a panel, and an approach for technical validation of multiplex panels which detects and addresses known risks.
Validating Antibodies for Research Applications in IHC
The importance of building a validation structure within your lab cannot be overstated. Once in place, the ability to identify high-quality specific results compared to spurious, non-specific antibody staining on tissue will be easier and allow the researcher to focus on downstream applications, such as single and multiplex IHC. This webinar will focus on the steps and challenges in antibody selection and validation to enable success and reproducibility in antibody staining in IHC.