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Aperio Cytoplasm Algorithm – Accurate Subcellular IHC Analysis

Aperio Cytoplasm Algorithm

The Aperio Cytoplasm Algorithm allows you to model and quantitate staining with a protein biomarker within the cytoplasm of cells. Use the Aperio Cytoplasm Algorithm to quantify staining area and intensity for cytoplasmic-specific markers, while also measuring translocation of staining into the cell nucleus, for a detailed picture of the cell immunohistochemistry.

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Get the full picture in your slides

The Aperio Cytoplasm Algorithm can automatically differentiate between staining in the nucleus and the cytoplasm. This insight on sub-cellular localization of proteins enables you to better understand cell processes.

User friendly interface

Aperio Image Analysis algorithms are designed with usability in mind. The Aperio Cytoplasm Algorithm has an intuitive set-up workflow, with instant feedback in the form of a visual tuning window, so you can see the effects of each parameter as you adjust.

Analyze dual-plex staining

Researchers need to get the most information possible from limited tissue samples. The Aperio Cytoplasm Algorithm allows use of a counterstain, and up to two positive stains, allowing you to get a greater amount of accurate, quantitative data from your slides.

Tuned to your needs

The customizable algorithm inputs allow you to adjust the algorithm for a range of diverse, specific research applications. Save your settings as an analysis macro that can be easily re-run on batches of slides in Aperio eSlide Manager or Aperio Image Analysis Workstation.

Key download

 Brochure (pdf)

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Aperio RUO (Research Use Only) Image Analysis Algorithms have been validated by Leica Biosystems for use with .svs images from Aperio AT2, Aperio CS2, and Aperio VERSA scanners. Use of Aperio RUO Algorithms with other available scanners has not been validated, and Leica Biosystems cannot train or support customers in use of Aperio RUO Algorithms with images from these scanners.