http://www.leicabiosystems.com/ Article tagged with en-US A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared. http://www.leicabiosystems.com/pathologyleaders/an-introduction-to-decalcification/ Tue, 23 Oct 2012 00:00:00 +0200 Geoffrey Rolls Wilms’ Tumour (WT) is a tumour of the kidneys that typically occurs in children. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas. The NCL-L-WT1-562 (WT49) was found to be very specific and best suited to this facility’s fully automated Immunohistochemistry (IHC) laboratory, when compared with the WT1 6F-H2 clone. The positive reaction of WT49 was restricted to nuclear staining without cytoplasmic staining which has been described in other WT1 antibodies. http://www.leicabiosystems.com/pathologyleaders/a-technical-quandary-comparison-between-two-wilms-tumour-antibody-clones/ Wed, 20 Jun 2012 00:00:00 +0200 Dr Karen Brundyn, Rochelle van Wijk, Charlene M Williams The DOG-1 antibody can potentially diagnose approximately 13% more GIST’s on IHC than CD117 staining. It is a sensitive and specific marker for GIST, warranting further investigation for the inclusion of this marker as an adjuvant to the panel of antibodies for the accurate identification and diagnosis of GIST. http://www.leicabiosystems.com/pathologyleaders/dog-1-a-breed-showing-k9-excellence/ Mon, 28 May 2012 00:00:00 +0200 Dr Rustum Solomon, Rochelle van Wijk, Natasha Rossouw Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue processing, microtomy and staining, the morphological and histochemical information obtainable from the specimen will be compromised. This series of five articles explains the theory behind fixation and the practical use of common, emerging and specialized fixitives. http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-1-the-process-of-fixation-and-the-nature-of-fixatives/ Tue, 06 Mar 2012 15:10:00 +0100 Geoffrey Rolls This second part of the Fixation and Fixatives series covers the factors that influence the rate and effectiveness of tissue fixation as well as looking at two common fixatives: formaldehyde (histology) and glutaraldehyde (ultrastructural electron microscopy studies). http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-2-factors-influencing-chemical-fixation-formaldehyde-and-glutaraldehyde/ Tue, 06 Mar 2012 15:08:00 +0100 Geoffrey Rolls As well as formaldehyde and glutaraldehyde, discussed previously, a number of other reagents have been used for fixation, sometimes in simple solution but often combined with other agents in the form of compound fixatives. The more important of these are discussed here in Part 3 of the Fixation and Fixatives series. http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-3-fixing-agents-other-than-the-common-aldehydes/ Tue, 06 Mar 2012 14:00:00 +0100 Geoffrey Rolls In this fourth part of the Fixation and Fixatives series, we look at some of the many popular and traditional fixative solutions that have been used in histology for the last 100 years. This part also has an overview of proprietary solutions and provides advice on how to select the right fixative for your application. http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-4-popular-fixative-solutions/ Tue, 06 Mar 2012 13:00:00 +0100 Geoffrey Rolls In this final part of the Fixation and Fixatives series we look at practical ways to optimise fixation quality, discuss how heat effects fixation and finish with an introduction to the relatively new field of microwave fixation. http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-5-practical-procedures-to-optimise-quality-the-effects-of-heat-and-microwaves/ Tue, 06 Mar 2012 00:00:00 +0100 Geoffrey Rolls Join Clifford M. Chapman, MS, HTL(ASCP), QIHC, technical director, Strata Pathology Services Inc., Lexington, MA, on April 26 from 2-3 p.m. for a free webinar on quality management for the histology laboratory. http://www.leicabiosystems.com/pathologyleaders/webinar-quality-management-for-the-histology-laboratory/ Mon, 06 Feb 2012 00:00:00 +0100 Clifford Chapman The hematoxylin and eosin stain (H&E) is the most widely used stain in histology and histopathology laboratories. When it is properly performed it has the ability to demonstrate a wide range of normal and abnormal cell and tissue components and yet it is a relatively simple stain to carry out on paraffin or frozen sections. In histopathology a high proportion of cases can be diagnosed by an experienced pathologist using an H&E stain alone. http://www.leicabiosystems.com/pathologyleaders/performing-a-hematoxylin-and-eosin-stain-a-step-by-step-guide/ Tue, 17 Jan 2012 00:00:00 +0100 Geoffrey Rolls