in situ hybridization

• Article
  Immunohistochemistry and Molecular Testing – Co-evolving Roles
  

  31. May 2010 Author: Colin Tristram Institute: Leica Biosystems, Wetzlar, Germany

  As molecular techniques become routine throughout all Pathology disciplines, we reflect on the role of traditional IHC and its use in current and future diagnostic protocols. Read article
  
  
• Article
  Detection of Multiple Cell Molecules and mRNA
  

  01. Oct 2008 Authors: Teresa Marafioti, David Y. Mason, Erica Ballabio, Jennifer C. Paterson Institute: University of Oxford, John Radcliffe Hospital, Laboratory Sciences, United Kingdom

  Double immunoenzymatic staining is a widely used technique to directly analyse the expression of multiple molecules in a single tissue section. One of the aims of our work was to explore the capability of an automated staining system to perform multiple labeling. Read article
  
  
• Article
  Effects of Fixation and Tissue Processing on Immunocytochemistry
  

  01. Oct 2008 Author: Peter Jackson Institute: The General Infirmary at Leeds LS1 3EX, Department of Histopathology and Molecular Pathology, UK

  One of the first uses of formaldehyde as a fixative for tissue specimens was accidentally discovered by a Dr. F. Blum towards the end of the nineteenth century. Based on Blum’s observations a 4% solution has remained the percentage recommended for formaldehyde fixation and has not been challenged… Read article
  
  
• Article
  The Utility & Assessment of RNA ISH Probes for EBV, Kappa and Lambda on BOND™
  

  01. Oct 2008 Author: Adam Meeney Institute: St Thomas’ Hospital, Histopathology Department, London, United Kingdom

  Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that infects and establishes persistent infection in a host. Latent EBV infection has been linked to a wide range of benign and malignant lesions. This study compares the sensitivity of automated IHC and ISH staining to detect latent EBV… Read article
  
  
• Article
  The Identification of Kappa and Lambda in Multiple Myeloma Using Automated In-Situ Hybridisation Techniques
  

  15. Sep 2007 Author: Kay Elderfield, M.Sc. Institute: Hammersmith Hospital, Department of Molecular Diagnostics, London, UK

  Multiple myeloma is the second most frequent B-cell malignancy in Western countries. Current guidelines suggest that light chain clonality needs to be demonstrated to confirm diagnosis of multiple myeloma. Immunohistochemistry of kappa and lambda light chains can often give difficulties in… Read article
  
  
• Article
  In Situ Hybridisation for the Detection of Kappa and Lambda Light Chain mRNA
  

  15. Sep 2007 Institute: Newcastle University, Faculty of Medical Sciences, Newcastle upon Tyne, UK

  For most aspects of cell characterisation in haematopathology, histology combined with immunohistochemistry (IHC) provides the current gold-standard. Despite more than 20 years of continuous advancement in antigen retrieval and new reagents for IHC, it remains the case that detection of… Read article