in situ hybridization
Article01. Oct 2008 Institute: University of Oxford, John Radcliffe Hospital, Laboratory Sciences, United KingdomDouble immunoenzymatic staining is a widely used technique to directly analyse the expression of multiple molecules in a single tissue section. One of the aims of our work was to explore the capability of an automated staining system to perform multiple labeling.
Article01. Oct 2008 Institute: The General Infirmary at Leeds LS1 3EX, Department of Histopathology and Molecular Pathology, UKOne of the first uses of formaldehyde as a fixative for tissue specimens was accidentally discovered by a Dr. F. Blum towards the end of the nineteenth century. Based on Blum’s observations a 4% solution has remained the percentage recommended for formaldehyde fixation and has not been challenged…
Article01. Oct 2008 Institute: St Thomas’ Hospital, Histopathology Department, London, United KingdomEpstein-Barr virus (EBV) is a ubiquitous herpesvirus that infects and establishes persistent infection in a host. Latent EBV infection has been linked to a wide range of benign and malignant lesions. This study compares the sensitivity of automated IHC and ISH staining to detect latent EBV…
ArticleThe Identification of Kappa and Lambda in Multiple Myeloma Using Automated In-Situ Hybridisation Techniques15. Sep 2007 Institute: Hammersmith Hospital, Department of Molecular Diagnostics, London, UKMultiple myeloma is the second most frequent B-cell malignancy in Western countries. Current guidelines suggest that light chain clonality needs to be demonstrated to confirm diagnosis of multiple myeloma. Immunohistochemistry of kappa and lambda light chains can often give difficulties in…