Immunohistochemistry

• Tutorial
  An Animated Guide to Immunohistochemistry
  

  31. Aug 2011 Author: James Anderson Institute: Leica Biosystems, Wetzlar, Germany

  This tutorial illustrates the basic steps needed to create the immunohistochemistry (IHC) stains used in diagnosis of tissue-based disease. Read article
  
  
• Article
  HIG2 Protein: New Frontiers in Diagnosis
  

  02. May 2011 Authors: Hikmat A. Al-Ahmadie, Maria Tretiakova, Ph.D., MD Institutes: Memorial Sloan-Kettering Cancer Center, Department of Pathology, New York, USA, University of Chicago, Department of Pathology, Chicago, IL, USA

  Tumor hypoxia is a common phenomenon in some subtypes of renal cell carcinomas (RCC) and may play a role in their biology and response to treatment. Here we evaluate a novel hypoxia marker HIG2 in renal cortical tumors and normal tissues. Read article
  
  
• Article
  Technical Brief of Heat Induced Epitope Retrieval
  

  20. Apr 2011 Author: Russell Myers, Ph.D. Institute: Leica Biosystems, Wetzlar, Germany

  The value of immunohistochemistry (IHC) is due to the power of the procedure to recognize and localize specific proteins (markers) within a tissue specimen. By determining the presence or absence of specific markers within a tumor the pathologist is provided with clues that may be helpful in… Read article
  
  
• Article
  Immunohistochemistry and Molecular Testing – Co-evolving Roles
  

  31. May 2010 Author: Colin Tristram Institute: Leica Biosystems, Wetzlar, Germany

  As molecular techniques become routine throughout all Pathology disciplines, we reflect on the role of traditional IHC and its use in current and future diagnostic protocols. Read article
  
  
• Article
  Evaluation of Antibody to Folate Receptor-alpha (FR-α)
  

  31. May 2010 Authors: Paul Scorer, Nicola Lawson, Ph.D. Institute: Leica Biosystems, Wetzlar, Germany

  Folate Receptor-alpha (FR-alpha) has been described extensively in many tumour and tissue types. Up to now the majority of this work has been looking at mRNA with some limited immunohistochemistry restricted to frozen tissue. In this article we review the extensive testing undertaken to evaluate a… Read article
  
  
• Article
  3D Histopathology of the Liver Using Dual Chromogen Histochemistry
  

  15. May 2010 Authors: D Treanor, P Quirke, D Magee, D M Crellin, M Shires, Peter Jackson, S. Gray, A Ismail Institutes: University of Leeds, School of Computing, UK, Leeds Teaching Hospital's Trust, Histopathology Department, Leeds, UK, University of Leeds, Pathology and Tumor Biology, LIMM, UK

  Three dimensional reconstruction of liver tissue is beneficial as it can improve understanding of the normal architecture of the vascular and biliary system; additionally, awareness of how these systems are affected by various pathological processes may help to inform future treatment choices. Read article
  
  
• Article
  Immunohistochemical Analysis of Carbonic Anhydrase IX (CA IX) in Renal Cortical Tumors
  

  15. May 2010 Author: P Illei, MD Institute: Johns Hopkins University, Department of Pathology, Baltimore, MD, USA

  A subset of renal cortical tumors cannot be accurately classified based on their morphologic features owing to partial overlap in their histologic appearance. Read article
  
  
• Article
  CD99: Development and Evaluation of a Novel Monoclonal Antibody for use in Manual and Automated Immunohistochemistry
  

  15. May 2010 Authors: Gary McIntosh, Ph.D. , Michael Pinkney, Paul Scorer, Nigel Piggott, Ph.D. , Nicola Lawson, Ph.D. , Jeanette Doherty, Andrea Cleghorn Institutes: Leica Microsystems, Leica Biosystems, Wetzlar, Germany

  CD99 is a transmembrane glycoprotein which is expressed in a wide range of tissues. Although its function is not fully understood it has been implicated in a variety of cellular processes including the homotypic aggregation of the T cells, apoptosis of immature thymocytes, up-regulation of T cell… Read article
  
  
• Article
  Prospective Evaluation of New CD19, CD30 and CD7 Antibodies for Fixed Tissue Immunohistology
  

  10. May 2010 Authors: Gary McIntosh, Ph.D. , Colin Tristram, Paul Scorer, Nigel Piggott, Ph.D. , Stephen Cowell, G. Menon Institutes: Leica Biosystems, Wetzlar, Germany, Newcastle University, Newcastle upon Tyne Hospitals NHS Foundation Trust, UK, Northern Institute for Cancer Research (NICR), Newcastle, UK

  The North East Haematopathology Diagnostic Service, based at the Royal Victoria Infirmary in Newcastle upon Tyne, provides a regional service for expert assessment of lymphoproliferative and myeloproliferative disorders for a regional network of 14 NHS hospitals in the North East and Cumbria. Read article
  
  
• Article
  Brains and Brawn - Antibodies in Research and Diagnosis
  

  07. Sep 2009 Authors: Nigel Piggott, Ph.D. , Gary McIntosh, Ph.D. Institute: Leica Biosystems, Wetzlar, Germany

  The increased sensitivity of immunohistochemistry (IHC) over standard Hematoxylin and Eosin (H&E) staining has established the IHC technique in the study of many diseases. Herein we describe the utility of some NovocastraTM antibodies that can be used in the study of brain and muscle diseases. Read article
  
  
• Article
  Effects of Fixation and Tissue Processing on Immunocytochemistry
  

  01. Oct 2008 Author: Peter Jackson Institute: The General Infirmary at Leeds LS1 3EX, Department of Histopathology and Molecular Pathology, UK

  One of the first uses of formaldehyde as a fixative for tissue specimens was accidentally discovered by a Dr. F. Blum towards the end of the nineteenth century. Based on Blum’s observations a 4% solution has remained the percentage recommended for formaldehyde fixation and has not been challenged… Read article
  
  
• Article
  CD11c
  

  01. Oct 2008 Author: Wendy N. Erber Institute: Addenbrooke's Hospital, Haematology Department, Cambridge, United Kingdom

  CD11c is an integrin glycoprotein expressed by mononocytes and macrophages, neutrophils, myeloid dendritic cells and a small subset of lymphocytes. Although antibodies to CD11c have been available since the mid-1980s, the new antibody 5D11 is the first to recognise a formalin-resistant epitope. Read article
  
  
• Article
  CD33 Antigen Detection by PWS44
  

  01. Oct 2008 Authors: E Hyjek, Louise Flintoft, Nigel Piggott, Ph.D. , Mark Rees, Ph.D. , Elizabeth Udale, Victoria Reid, Ph.D. , Jeanette Doherty, Paul Scorer, Gary McIntosh, Ph.D. , A Chadburn, D M Knowles, I I Bansal Institutes: Leica Biosystems, Wetzlar, Germany, Weill Cornell Medical College, Department of Pathology & Laboratory Medicine, New York, NY, USA

  CD33 Antigen Detection by PWS44 a New Monoclonal Antibody Reactive in Paraffin Tissues Sections: Pattern of Reactivity and Potential Diagnostic Utility. Read article
  
  
• Article
  The Utility & Assessment of RNA ISH Probes for EBV, Kappa and Lambda on BOND™
  

  01. Oct 2008 Author: Adam Meeney Institute: St Thomas’ Hospital, Histopathology Department, London, United Kingdom

  Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that infects and establishes persistent infection in a host. Latent EBV infection has been linked to a wide range of benign and malignant lesions. This study compares the sensitivity of automated IHC and ISH staining to detect latent EBV… Read article
  
  
• Tutorial
  From Concept to Diagnosis – How Antibodies are Designed
  

  08. May 2008 Author: Kenneth Mitchell, Dr. Institute: Leica Biosystems, Wetzlar, Germany

  Newcastle upon Tyne – this lively town in Northern England is not only famous for its culture, but it has been the base for the developer and manufacturer of antibodies and other reagents of the brand, Novocastra™. “Novocastra” is based upon the Latin name for the city of Newcastle and was the name… Read article
  
  
• Article
  CD3 Mouse Monoclonal Antibodies, Assessment of the New Clone LN10 Against PS1: An Improvement on a Routine Tool for the Haematopathologist!
  

  15. Sep 2007 Authors: Harald Stein, Korinna Jöhrens, Ioannis Anagnostopoulos Institute: Charité Hospital, Institute of Pathology and Reference Center for Haematopathology, Berlin, Germany

  CD3 is a reliable and specific marker for the detection of cells of the T-cell lineage and has been, therefore, routinely used by pathologists for many years. The PS1 clone was a great advancement when it was first released. It was robust and able to work in decalcified bone marrow trephines where… Read article
  
  
• Article
  CD3: And Another Thing...
  

  15. Sep 2007 Author: Philippa Munson Institute: University College London Hospital, UCL Advanced Diagnostics, Department of Pathology, London, UK

  We at UCL Advanced Diagnostics were lucky enough to be involved in the external assessment of CD3, LN10 in its validation stage. This enabled us to begin using it routinely as soon as it was commercially available, indicating our feelings on it! Read article
  
  
• Article
  Factor XIIIa Expression in Normal and Pathological Skin
  

  15. Sep 2007 Authors: Catherine Stefanato, Ph.D. , Guy Orchard, Ph.D. Institute: St Thomas’ Hospital, Histopathology Department, London, United Kingdom

  FXIIIa is a blood and intracellularly produced coagulation factor, which is widely expressed in variety of cell types. It is believed to be vital in the final stages of the clotting cascade and is also important in wound healing and repair mechanisms. It also has a role in angiogenesis in embryo… Read article
  
  
• Article
  Bcl-6 Immunostaining – Practical Aspects in a Clinical Diagnostic Setting
  

  01. Sep 2007 Author: Alan Ramsay, Dr., MD Institute: University College London Hospitals, Histopathology Department, London UK

  The bcl-6 gene is associated with translocations affecting 3q27 in cases of diffuse large B-cell lymphoma (DLBCL). The gene product is a zinc finger protein thought to be involved in control of germinal centre proliferation. Bcl-6 protein can be detected immunohistochemically and positive staining… Read article
  
  
• Article
  First CD33 Antibody Effective in FFPE Tissue - New reagent to detect acute myeloid leukemias
  

  01. Sep 2007 Author: Kenneth Mitchell, Dr. Institute: Leica Biosystems, Wetzlar, Germany

  Detection of CD33 using monoclonal antibodies has been critical in immunophenotyping acute leukemias, particularly acute myeloid leukemias. To date this has only been possible by either flow cytometry or by frozen section immunohistochemistry. Now the first CD33 antibody... Read article