Advancing Cancer Diagnostics
Improving Lives
Warning! You won't be able to use the quotation basket until you enable cookies in your Web browser.
Warning! Your Web browser is no longer supported. Please upgrade to a modern browser.

Effects of decalcification on In Situ Hybridization Stains and Immunohistochemical Stains

PACE credits are no longer available for webinars more than 6 months old.


Publications describing the effects of decalcification on immunohistochemistry (IHC) staining have been published intermittently with limited new data since the 1990s.  At the same time IHC stains have become an ever more important and dependent tool in anatomic pathology for diagnosis, prognosis, and treatment purposes. Current instrumentation, in addition to being fully automated, typically use polymer based detection systems, in place of avidin-biotin complex based detection, which was predominate when most of the previous studies were performed. This presentation will review performance of ISH and IHC markers decalcification reagents, and antibodies with current detection system of polymer based detection.   Also, a review of current IHC validation recommendation concerning decalcified tissue will be discussed.

Learning Objectives:

  1. Characterize performance of ISH markers; kappa and lambda, with current decalcification reagents will be reviewed.
  2. Characterize performance of 33 different IHC markers with current decalcification reagents will be reviewed.
  3. Review current IHC validation recommendations concerning decalcified tissue currently in the USA.

Leica Biosystems webinars, training presentations and related materials provide general information regarding particular subjects and are not intended to be, and should not be construed as medical, regulatory or legal advice. The views and opinions expressed are the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents.

For the use of any product, the product information guides, inserts and operation manuals of the various products and devices should be consulted. Leica Biosystems and the editors disclaim any liability arising directly or indirectly from the use of devices, techniques or procedures described in these materials.

Copyright © 2016 by Leica Biosystems Richmond Inc. All rights reserved.LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.