Difficult Blocks and Reprocessing

Author:
Institute: Leica Biosystems, Wetzlar, Germany
18. October 2011

What To Do When Your Block Won’t Section

From time to time, in all histopathology laboratories, paraffin blocks will be encountered that are difficult or even impossible to section. This book provides guidance on how to deal with these difficult blocks to get the best possible section, and how to identify and avoid a repeat of underlying causes.

Part of the Topic
Tagged as
Share

Chapters

Part 1: The initial assessment
Why is my block difficult or impossible to cut?

Part 2: Getting more information
Examples of typical problems and their causes

Part 3: Detailed Solutions
Detailed solutions for difficult blocks

Part 4: Root Causes
The root cause of processing problems and their prevention

Part 5: Validation
How to validate a reprocessing method

Part 6: Appendix
Reprocessing decision trees
Tables
References

Comments

Geoffrey Rolls

Geoffrey Rolls, Histology Consultant, Leica Biosystems, Melbourne, Australia, has extensive experience practicing and teaching histology and histotechnology including 30 years as Senior Lecturer in the Department of Laboratory Medicine at RMIT University in Melbourne, Australia. He holds a science degree and a professional Fellowship of the Australian Institute of Medical Scientists. He has a broad interest in histotechnology, particularly in the areas of tissue processing and microtomy and in general histology education. For the last ten years he has acted as a consultant to Leica Biosystems.

Geoffrey.Rolls@leicabiosystems.com

Related Products

Leica RM2255
Leica ASP6025 Automated Vacuum Tissue Processor Leica RM2255 Fully Automated Rotary Microtome