Geoffrey Rolls
Geoffrey Rolls, Histology Consultant, Leica Biosystems, Melbourne, Australia, has extensive experience practicing and teaching histology and histotechnology including 30 years as Senior Lecturer in the Department of Laboratory Medicine at RMIT University in Melbourne, Australia. He holds a science degree and a professional Fellowship of the Australian Institute of Medical Scientists. He has a broad interest in histotechnology, particularly in the areas of tissue processing and microtomy and in general histology education. For the last ten years he has acted as a consultant to Leica Biosystems.
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Article
23. Oct 2012 Institute: Leica Biosystems, Wetzlar, GermanyA discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared. Read article
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Article
06. Mar 2012 Institute: Leica Biosystems, Wetzlar, GermanyFixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue processing, microtomy and staining, the… Read article
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Article
06. Mar 2012 Institute: Leica Biosystems, Wetzlar, GermanyThis second part of the Fixation and Fixatives series covers the factors that influence the rate and effectiveness of tissue fixation as well as looking at two common fixatives: formaldehyde (histology) and glutaraldehyde (ultrastructural electron microscopy studies). Read article
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Article
06. Mar 2012 Institute: Leica Biosystems, Wetzlar, GermanyAs well as formaldehyde and glutaraldehyde, discussed previously, a number of other reagents have been used for fixation, sometimes in simple solution but often combined with other agents in the form of compound fixatives. The more important of these are discussed here in Part 3 of the Fixation and… Read article
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Article
06. Mar 2012 Institute: Leica Biosystems, Wetzlar, GermanyIn this fourth part of the Fixation and Fixatives series, we look at some of the many popular and traditional fixative solutions that have been used in histology for the last 100 years. This part also has an overview of proprietary solutions and provides advice on how to select the right… Read article
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Article
06. Mar 2012 Institute: Leica Biosystems, Wetzlar, GermanyIn this final part of the Fixation and Fixatives series we look at practical ways to optimise fixation quality, discuss how heat effects fixation and finish with an introduction to the relatively new field of microwave fixation. Read article
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Tutorial
17. Jan 2012 Institute: Leica Biosystems, Wetzlar, GermanyThe hematoxylin and eosin stain (H&E) is the most widely used stain in histology and histopathology laboratories. When it is properly performed it has the ability to demonstrate a wide range of normal and abnormal cell and tissue components and yet it is a relatively simple stain to carry out on… Read article
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Tutorial
18. Oct 2011 Institute: Leica Biosystems, Wetzlar, GermanyFrom time to time, in all histopathology laboratories, paraffin blocks will be encountered that are difficult or even impossible to section. This publication provides guidance on how to deal with these difficult blocks to get the best possible section, and how to identify and avoid a repeat of… Read article
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Tutorial
30. May 2011 Institute: Leica Biosystems, Wetzlar, GermanyAn introduction to the preparation of specimens for microscopy in the histopathology laboratory. Read article
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Tutorial
26. May 2011 Institute: Leica Biosystems, Wetzlar, GermanyMicroscopic analysis of cells and tissues requires the preparation of very thin, high quality sections (slices) mounted on glass slides and appropriately stained to demonstrate normal and abnormal structures. Most fresh tissue is very delicate and easily distorted and damaged and it is thus… Read article
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Tutorial
01. Mar 2010 Institute: Leica Biosystems, Wetzlar, GermanyCreating great paraffin sections using a rotary microtome takes a great deal of skill and experience. Microtomy and Paraffin Section Preparation is a great training aid for new microtomists and is an excellent refresher for experienced operators. Read article
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Tutorial
16. Jun 2008 Institute: Leica Biosystems, Wetzlar, GermanyFrom patient to pathologist, preparing tissue specimens for histological examination requires care, skill and sound procedures. This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors. Read article
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Tutorial
01. Apr 2008 Institute: Leica Biosystems, Wetzlar, GermanyHistology artifacts and cytology artifacts are structures that were not originally present in the living tissue. These artifacts can be from a range of sources including contamination, poor tissue preparation and surgical procedures. Understanding artifacts is important for both histologists and… Read article