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Mycobacterium tuberculosis Primer Sets

Mycobacterium tuberculosis Novocastra

Agarose gel: electrophoresis of PCR products using NCL-MT-PS. Lane A, 123bp ladder (Sigma). Lane B, internal control only. Lanes C, D and E, Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium intracellulare isolates, respectively. Lanes F and G, Mycobacterium tuberculosis-positive human sputa. Lane H, Mycobacterium tuberculosis-positive, formalin-fixed, paraffin-embedded human lung. The polymerase chain reaction (PCR) process is covered by patents owned by Hoffman Laroche Inc. and Hoffman La-Roche Ltd. Purchase of these products does not carry a licence to perform the PCR process.
Agarose gel: electrophoresis of PCR products using NCL-MT-PS. Lane A, 123bp ladder (Sigma). Lane B, internal control only. Lanes C, D and E, Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium intracellulare isolates, respectively. Lanes F and G, Mycobacterium tuberculosis-positive human sputa. Lane H, Mycobacterium tuberculosis-positive, formalin-fixed, paraffin-embedded human lung. The polymerase chain reaction (PCR) process is covered by patents owned by Hoffman Laroche Inc. and Hoffman La-Roche Ltd. Purchase of these products does not carry a licence to perform the PCR process.

Antigen Background

The Mycobacterium tuberculosis primer set, NCL-MT-PS, consists of a pair of oligonucleotide primers (MT-1 and MT-2) which amplify the 123bp insertion sequence IS6110.

The sequence is unique to members of the Mycobacterium tuberculosis complex (MTBC) and is present as a sequence in multiple copies in many common isolates.

Also provided is an internal control DNA for aiding in the detection of false-negative PCR results.


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Code Name Configuration Use Datasheet MSDS Qty
MT-PS NCL-MT-PS Primer Set O Info MSDS