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Corynebacterium diphtheriae Primer Sets

Corynebacterium diphtheriae Novocastra

Agarose gel: electrophoresis of PCR products using NCL-CD-PS. Lane A, 100bp ladder (Promega). Lane B, Reagent blank. Lanes C-F and H, five toxigenic Corynebacterium diphtheriae isolates. Lane G, non-toxigenic Corynebacterium diphtheriae isolate. The polymerase chain reaction (PCR) process is covered by patents owned by Hoffman Laroche Inc. and Hoffman La-Roche Ltd. Purchase of these products does not carry a licence to perform the PCR process.
Agarose gel: electrophoresis of PCR products using NCL-CD-PS. Lane A, 100bp ladder (Promega). Lane B, Reagent blank. Lanes C-F and H, five toxigenic Corynebacterium diphtheriae isolates. Lane G, non-toxigenic Corynebacterium diphtheriae isolate. The polymerase chain reaction (PCR) process is covered by patents owned by Hoffman Laroche Inc. and Hoffman La-Roche Ltd. Purchase of these products does not carry a licence to perform the PCR process.

Antigen Background

Large outbreaks of diphtheria in eastern Europe and modern travel have led to an increased awareness of Corynebacterium diphtheriae. Most western isolates are still non-toxigenic, but toxin tests require experience and expertise.The Corynebacterium diphtheriae primer set, NCL-CD-PS, consists of a pair of oligonucleotide primers (CD-1 and CD-2) which amplify a 246bp region of the toxin gene alpha subunit.

Also provided is an internal control DNA for aiding in the detection of false-negative PCR results. The internal control DNA may be added to the test PCR, where it will amplify with the same primers but yield a product of different size.


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Code Name Configuration Use Datasheet MSDS Qty
CD-PS NCL-CD-PS Primer Set O Info MSDS