Corynebacterium diphtheriae Novocastra
Large outbreaks of diphtheria in eastern Europe and modern travel have led to an increased awareness of Corynebacterium diphtheriae. Most western isolates are still non-toxigenic, but toxin tests require experience and expertise.The Corynebacterium diphtheriae primer set, NCL-CD-PS, consists of a pair of oligonucleotide primers (CD-1 and CD-2) which amplify a 246bp region of the toxin gene alpha subunit.
Also provided is an internal control DNA for aiding in the detection of false-negative PCR results. The internal control DNA may be added to the test PCR, where it will amplify with the same primers but yield a product of different size.