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Proliferating Cell Nuclear Antigen Primary Antibodies

Proliferating Cell Nuclear Antigen Novocastra

Human seminoma: immunohistochemical staining for proliferating cell nuclear antigen using NCL-PCNA. Note nuclear staining of proliferating tumor cells. Paraffin section.
Human seminoma: immunohistochemical staining for proliferating cell nuclear antigen using NCL-PCNA. Note nuclear staining of proliferating tumor cells. Paraffin section.

Antigen Background

Proliferating cell nuclear antigen (PCNA) is a 36 kD protein which is highly conserved between species. PCNA functions as a co-factor for DNA polymerase delta in S phase and also during DNA synthesis associated with DNA damage repair mechanisms. The PCNA molecule has a half-life in excess of 20 hours, and therefore, may be detected in non-cycling cells eg those in G0 phase.

Product Specific Information

Heat induced epitope retrieval using 10mM citrate buffer (pH6.0) may improve staining on overfixed tissues, but due to increased sensitivity using this technique, care must be taken with the interpretation of results. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air drying overnight onto 3-aminopropyltriethoxysilane (Apes) coated slides may produce improved results.


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Code Name Configuration Use Datasheet MSDS Qty
PCNA 1ml NCL-PCNA Lyophilised Concentrated Monoclonal Antibody C, P, W Info MSDS
PCNA-L-U 1ml NCL-L-PCNA US IVD Lyophilised Concentrated Monoclonal Antibody C, P, W Info MSDS
PCNA-S 0.1ml NCL-PCNA Lyophilised Concentrated Monoclonal Antibody C, P, W Info MSDS