Aurora Kinase 2 Novocastra
Aurora Kinase 1 and 2 encode cell cycle-regulated serine/threonine kinases that are involved in microtubule spindle activities during mitosis and meiosis. Aurora Kinase 2, also known as AurkA, STK15, BTAK, ARK1 and AIK1, localizes to interphase and mitotic centrosomes and to the spindle poles.
It is degraded rapidly after G2/M phase release in mammalian cells. Aurora Kinase 2 is reported to be expressed at high levels in testis and various proliferating cell lines, including HeLa cells. Aurora Kinase 2 is regulated by phosphorylation which is important both for its activity and stability.
The inhibition of its activity leads to the formation of a monopolar spindle because its activity is necessary for centrosome separation. Aurora Kinase 2 overexpression leads to centrosome amplification, chromosome instability and transformation in mammalian cells. Overexpression of both active and inactive Aurora Kinase 2 can lead to polyploidy.
This suggests that Aurora Kinase 2 can behave as a dominant negative mutant and inhibit other aurora kinases. When inactive kinase is expressed, however, the cells eventually die and do not become immortalized, unlike with the active kinase.