Whole Chromosome

Introduction

 

The Kreatech™ Whole Chromosome probes hybridize to unique sequences spanning the entire length of the target chromosome.

These probes are derived from flow-sorted or microdissected chromosomes and provide accurate coverage with excellent signal specificity and high fluorescent intensity. Some minor cross-hybridization may occur at the short arm of acrocentric chromosomes (13, 14, 15, 21 and 22) and in the pseudo-autosomal regions of chromosome X and Y.

Technical Information

 

The WC probes are supplied in a 5-test, 5x concentrated format. Hybridization buffer can be purchased separately (see General Purpose Reagents). The WC probes labeled in a choice of green (PlatinumBright™ 495) and red (PlatinumBright™ 550) or in blue (PlatinumBright™ 415) (for WC in blue please inquire).

 

Note:
Due to decondensation of chromosomal DNA signals on interphase, cells can be very diffuse. We therefore recommend not to use WC probes for interphase FISH.

 

"Our REPEAT-FREE™ Whole Chromosome ASR Probes in the table below are provided in a 10 µl, 5x concentrated format".

ASR - Analyte Specific Reagent